Special Section on Quantitative Phase Imaging in Biomedicine

Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood

[+] Author Affiliations
HyunJoo Park, Kyoohyun Kim, Sangyun Lee, YongKeun Park

Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea

Taegyu Ahn, Song-yi Kook, Dongheon Lee, Sunghun Na

Kangwon National University, Department of Obstetrics and Gynecology, Kangwon National University Hospital, School of Medicine, Chuncheon 200-701, Republic of Korea

In Bum Suh

Kangwon National University, Department of Laboratory Medicine, Kangwon National University Hospital, School of Medicine, Chuncheon 200-701, Republic of Korea

J. Biomed. Opt. 20(11), 111208 (Aug 11, 2015). doi:10.1117/1.JBO.20.11.111208
History: Received March 29, 2015; Accepted July 8, 2015
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Abstract.  Red blood cells (RBCs) from the cord blood of newborn infants have distinctive functions in fetal and infant development. To systematically investigate the biophysical characteristics of individual cord RBCs in newborn infants, a comparative study was performed on RBCs from the cord blood of newborn infants and from adult mothers or nonpregnant women using optical holographic microtomography. Optical measurements of the distributions of the three-dimensional refractive indices and the dynamic membrane fluctuations of individual RBCs were used to investigate the morphological, biochemical, and mechanical properties of cord, maternal, and adult RBCs at the individual cell level. The volume and surface area of the cord RBCs were significantly larger than those of the RBCs from nonpregnant women, and the cord RBCs had more flattened shapes than that of the RBCs in adults. In addition, the hemoglobin (Hb) content in the cord RBCs from newborns was significantly higher. The Hb concentration in the cord RBCs was higher than that in the nonpregnant women or maternal RBCs, but they were within the physiological range of adults. Interestingly, the amplitudes of the dynamic membrane fluctuations in cord RBCs were comparable to those in nonpregnant women and maternal RBCs, suggesting that the deformability of cord RBCs is similar to that of healthy RBCs in adults.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

HyunJoo Park ; Taegyu Ahn ; Kyoohyun Kim ; Sangyun Lee ; Song-yi Kook, et al.
"Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood", J. Biomed. Opt. 20(11), 111208 (Aug 11, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.111208


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