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17 August 2015 Quantification of light attenuation in optically cleared mouse brains
Angela d’Esposito, Daniil Nikitichev, Adrien Desjardins, Simon Walker-Samuel, Mark F. Lythgoe
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Journal of Biomedical Optics, Vol. 20, Issue 8, 080503 (August 2015). https://doi.org/10.1117/1.JBO.20.8.080503
Abstract
Optical clearing, in combination with recently developed optical imaging techniques, enables visualization and acquisition of high-resolution, three-dimensional images of biological structures deep within the tissue. Many different approaches can be used to reduce light absorption and scattering within the tissue, but there is a paucity of research on the quantification of clearing efficacy. With the use of a custom-made spectroscopy system, we developed a way to quantify the quality of clearing in biological tissue and applied it to the mouse brain. Three clearing techniques were compared: BABB (1:2 mixture of benzyl alcohol and benzyl benzoate, also known as Murray’s clear), pBABB (peroxide BABB, a modification of BABB which includes the use of hydrogen peroxide), and passive CLARITY. We found that BABB and pBABB produced the highest degree of optical clearing. Furthermore, the approach allows regional measurement of light attenuation to be performed, and our results show that light is most attenuated in regions with high lipid content. We provide a way to choose between the multiple clearing protocols available, and it could prove useful for evaluating images that are acquired with cleared tissues.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Angela d’Esposito, Daniil Nikitichev, Adrien Desjardins, Simon Walker-Samuel, and Mark F. Lythgoe "Quantification of light attenuation in optically cleared mouse brains," Journal of Biomedical Optics 20(8), 080503 (17 August 2015). https://doi.org/10.1117/1.JBO.20.8.080503
Published: 17 August 2015
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Cited by 18 scholarly publications.
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KEYWORDS
Brain
Tissue optics
Signal attenuation
Optical clearing
Tissues
Absorption
Spectroscopy
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