Special Section on Protein Photonics for Imaging, Sensing, and Manipulation: Honoring Prof. Osamu Shimomura, a Pioneer of Photonics for Biomedical Research

Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans

[+] Author Affiliations
Tomoki Nishiguchi, Toshimichi Yamada, Yusuke Nasu, Mashiho Ito, Hideaki Yoshimura, Takeaki Ozawa

The University of Tokyo, School of Science, Department of Chemistry, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

J. Biomed. Opt. 20(10), 101205 (Aug 27, 2015). doi:10.1117/1.JBO.20.10.101205
History: Received April 3, 2015; Accepted July 31, 2015
Text Size: A A A

Abstract.  Luciferase, a bioluminescent protein, has been used as an analytical tool to visualize intracellular phenomena. Luciferase with red light emission is particularly useful for bioluminescence imaging because of its high transmittance in mammalian tissues. However, the luminescence intensity of existing luciferases with their emission over 600 nm is insufficient for imaging studies because of their weak intensities. We developed mutants of Emerald luciferase (Eluc) from Brazilian click beetle (Pyrearinus termitilluminans), which emits the strongest bioluminescence among beetle luciferases. We successively introduced four amino acid mutations into the luciferase based on a predicted structure of Eluc using homology modeling. Results showed that quadruple mutations R214K/H241K/S246H/H347A into the beetle luciferase emit luminescence with emission maximum at 626 nm, 88-nm red-shift from the wild-type luciferase. This mutant luciferase is anticipated for application in in vivo multicolor imaging in living samples.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Tomoki Nishiguchi ; Toshimichi Yamada ; Yusuke Nasu ; Mashiho Ito ; Hideaki Yoshimura, et al.
"Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans", J. Biomed. Opt. 20(10), 101205 (Aug 27, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.101205


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.