Research Papers: Imaging

Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors

[+] Author Affiliations
Satoru Onoe, Masahiro Ono, Hideo Saji

Kyoto University, Graduate School of Pharmaceutical Sciences, Department of Patho-Functional Bioanalysis, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

Takashi Temma

Kyoto University, Graduate School of Pharmaceutical Sciences, Department of Patho-Functional Bioanalysis, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

National Cerebral and Cardiovascular Center Research Institute, Department of Investigative Radiology, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan

Kengo Kanazaki

Kyoto University, Graduate School of Pharmaceutical Sciences, Department of Patho-Functional Bioanalysis, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

Canon Inc., Corporate R&D Headquarters, Medical Imaging Project, 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan

J. Biomed. Opt. 20(9), 096006 (Sep 10, 2015). doi:10.1117/1.JBO.20.9.096006
History: Received April 11, 2015; Accepted July 15, 2015
Text Size: A A A

Abstract.  Photoacoustic imaging (PAI) contributes to tumor diagnosis through the use of PAI probes that effectively accumulate in tumors. Previously, we developed a symmetrical cyanine dye, IC7-1-Bu, which showed high potential as a PAI probe because of its high tumor targeting ability and sufficient in vivo PA signal. However, IC7-1-Bu lacks photostability for multiple laser irradiations, so we developed stabilized PAI probes using IC7-1-Bu as a lead compound. We focused on the effect of singlet oxygen (O12) generated by excited PAI probes on probe degeneration. We introduced a triplet-state quencher (TSQ) moiety into IC7-1-Bu to quench O12 generation and designed three IC-n-T derivatives with different linker lengths (n indicates linker length). The IC-n-T derivatives emitted in vitro PA signals that were comparable to IC7-1-Bu and significantly reduced O12 generation while showing improved photostability against multiple irradiations. Of the three derivatives evaluated, IC-5-T accumulated in tumors effectively to allow clear PAI of tumors in vivo. Furthermore, the photostability of IC-5-T was 1.5-fold higher than that of IC7-1-Bu in in vivo sequential PAI. These results suggest that IC-5-T is a potential PAI probe for in vivo sequential tumor imaging.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Satoru Onoe ; Takashi Temma ; Kengo Kanazaki ; Masahiro Ono and Hideo Saji
"Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors", J. Biomed. Opt. 20(9), 096006 (Sep 10, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.9.096006


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.