Neuronal activity occurs on a millisecond time scale across cell circuits distributed over the entire nervous system. Capturing these spatiotemporal patterns at adequate sampling rates to uncover the underlying principles of neuronal information processing is a grand technical challenge for systems neuroscience. Recently, it became possible in transparent reporter zebrafish larvae expressing genetically encoded fluorescent calcium indicators (GECIs)1,2 to detect calcium transients noninvasively in the whole nervous system. In this vertebrate model organism, imaging can thus deliver information about a close correlate of neuronal activation, calcium fluxes, at much higher spatial resolution than is achievable with electrophysiological point recordings. To exceed the speed of confocal or multiphoton laser scanning methods,3 light sheet microscopy methods have recently been developed4–6 that image an entire plane through the object of interest at once, such that about neurons in an entire zebrafish larval brain can be scanned at a frequency of about 1 Hz.4 Volumetric acquisition can be accelerated even further by light field imaging, an elegant method that avoids having to scan the object but instead reconstructs it volumetrically from multiple views acquired simultaneously from many different angles. This imaging method has recently been adapted for microscopy by using arrays of microlenses projecting to subregions of sufficiently sensitive and large image sensors.7,8 Light field microscopy (also known as plenoptic microscopy) could thus, in principle, capture the majority of fluorescent neurons in the brain of a reporter zebrafish in a single acquisition without the need for interpolation to correct for time delays. Neuroimaging of reporter zebrafish would benefit directly from such a gain in imaging speed. For instance, state-of-the-art fast light sheet microscopy, operating at about one volume per second, still misses a substantial fraction of calcium fluctuations detected by sensors such as GCaMP5G (rise times of and decay rates of 1,4). For faster fluorescent sensors, such as genetically encoded voltage sensors,9 even higher frame rates10 would be required for adequate sampling. Furthermore, fast volumetric imaging could simultaneously extract information from fluorescent molecular sensors together with “biomechanical” data from freely moving zebrafish larvae exhibiting unrestrained behavior; this may complement virtual environment approaches that investigate neural activity during fictive behavior in immobilized zebrafish larvae.11 Lately, technical advances have been made in acquisition and reconstruction of light field microscopy data12–14 and three light field microscopes have been custom-built in different laboratories specifically to image neuronal activity in immobilized Caenorhabditis elegans worms and zebrafish larvae.8,14,15 Despite the generous provision of open access documentation on light field microscopy hardware and open source software,16,17 an “off-the shelf” light field camera system would certainly help to disseminate this comparably straightforward and compact imaging technology for widespread biological use. In this work, we thus investigated the performance of commercially available multifocus plenoptic cameras18 (Raytrix GmbH) for fluorescence neuroimaging of zebrafish larvae.