JBO Letters

Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant

[+] Author Affiliations
Andrew L. Lopez, III, Shang Wang, Irina V. Larina

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United States

Kirill V. Larin

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston 77030, United States

University of Houston, Department of Biomedical Engineering, 3605 Cullen Boulevard, Houston 77204, United States

Samara State Aerospace University, 34 Moskovskoye Shosse, Samara 443086, Russia

Paul A. Overbeek

Baylor College of Medicine, Department of Molecular & Cellular Biology, One Baylor Plaza, Houston 77030, United States

J. Biomed. Opt. 20(9), 090501 (Sep 21, 2015). doi:10.1117/1.JBO.20.9.090501
History: Received July 8, 2015; Accepted August 14, 2015
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Abstract.  Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between mutant and control embryos. Our results indicate that live 4-D OCT imaging provides a powerful phenotyping approach to characterize embryonic cardiac function in mouse models.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Andrew L. Lopez, III ; Shang Wang ; Kirill V. Larin ; Paul A. Overbeek and Irina V. Larina
"Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant", J. Biomed. Opt. 20(9), 090501 (Sep 21, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.9.090501


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