Research Papers: General

Terahertz spectroscopy of oligonucleotides in aqueous solutions

[+] Author Affiliations
Mingjie Tang, Dongshan Wei, Chunlei Du

Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 400714, China

Qing Huang, Wei-ling Fu

Third Military Medical University, Southwest Hospital, Department of Laboratory Medicine, Chongqing 400038, China

Guozhong Zhao, Kuan Kou

Capital Normal University, Department of Physics, Beijing 100048, China

Tianying Chang, Hong-Liang Cui

Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing Key Laboratory of Multi-Scale Manufacturing Technology, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 400714, China

Jilin University, College of Instrumentation Science and Electrical Engineering, Changchun, Jilin 130061, China

Min Wang

Jilin University, College of Instrumentation Science and Electrical Engineering, Changchun, Jilin 130061, China

J. Biomed. Opt. 20(9), 095009 (Sep 21, 2015). doi:10.1117/1.JBO.20.9.095009
History: Received May 5, 2015; Accepted August 17, 2015
Text Size: A A A

Abstract.  A terahertz (THz) spectroscopic study is carried out to analyze DNA mutations in a label-free manner. Three newly designed liquid sample cells are considered and the best is selected as the sample carrier for THz transmission spectroscopic analyses. Discrimination based on spectral signatures of single-base mutations on single-stranded 20 nt oligonucleotides has been shown possible experimentally. The results clearly attest the ability of this promising approach for label-free analyses of single-base mutations of DNA molecules. This study has demonstrated that the THz spectroscopic technology can be considered as a potential diagnostic tool for investigating molecular reactions, such as DNA mutations.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Mingjie Tang ; Qing Huang ; Dongshan Wei ; Guozhong Zhao ; Tianying Chang, et al.
"Terahertz spectroscopy of oligonucleotides in aqueous solutions", J. Biomed. Opt. 20(9), 095009 (Sep 21, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.9.095009


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.