Special Section on Quantitative Phase Imaging in Biomedicine

Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing

[+] Author Affiliations
Pinhas Girshovitz, Irena Frenklach, Natan T. Shaked

Tel Aviv University, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv 69978, Israel

J. Biomed. Opt. 20(11), 111217 (Oct 06, 2015). doi:10.1117/1.JBO.20.11.111217
History: Received March 17, 2015; Accepted August 20, 2015
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Abstract.  We propose a new portable imaging configuration that can double the field of view (FOV) of existing off-axis interferometric imaging setups, including broadband off-axis interferometers. This configuration is attached at the output port of the off-axis interferometer and optically creates a multiplexed interferogram on the digital camera, which is composed of two off-axis interferograms with straight fringes at orthogonal directions. Each of these interferograms contains a different FOV of the imaged sample. Due to the separation of these two FOVs in the spatial-frequency domain, they can be fully reconstructed separately, while obtaining two complex wavefronts from the sample at once. Since the optically multiplexed off-axis interferogram is recorded by the camera in a single exposure, fast dynamics can be recorded with a doubled imaging area. We used this technique for quantitative phase microscopy of biological samples with extended FOV. We demonstrate attaching the proposed module to a diffractive phase microscopy interferometer, illuminated by a broadband light source. The biological samples used for the experimental demonstrations include microscopic diatom shells, cancer cells, and flowing blood cells.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Pinhas Girshovitz ; Irena Frenklach and Natan T. Shaked
"Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing", J. Biomed. Opt. 20(11), 111217 (Oct 06, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.111217


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