Research Papers: Imaging

Augmented microscopy: real-time overlay of bright-field and near-infrared fluorescence images

[+] Author Affiliations
Jeffrey R. Watson, Christian F. Gainer, Marek Romanowski

University of Arizona, Department of Biomedical Engineering, 1657 E. Helen Street, Tucson, Arizona 85721, United States

Nikolay Martirosyan, Jesse Skoch, G. Michael Lemole, Jr., Rein Anton

University of Arizona, Division of Neurosurgery, Department of Surgery, 1501 N. Campbell Avenue, Tucson, Arizona 85721, United States

J. Biomed. Opt. 20(10), 106002 (Oct 06, 2015). doi:10.1117/1.JBO.20.10.106002
History: Received June 29, 2015; Accepted September 2, 2015
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Abstract.  Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed NIR fluorescence (synthetic) images are merged within the optical path of a stereomicroscope. Under luminance of 100,000 lx, representing typical illumination of the surgical field, the augmented microscope detects 189 nM concentration of indocyanine green and produces a composite of the real and synthetic images within the eyepiece of the microscope at 20 fps. Augmentation described here can be implemented as an add-on module to visualize NIR contrast agents, laser beams, or various types of electronic data within the surgical microscopes commonly used in neurosurgical, cerebrovascular, otolaryngological, and ophthalmic procedures.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Jeffrey R. Watson ; Christian F. Gainer ; Nikolay Martirosyan ; Jesse Skoch ; G. Michael Lemole, Jr., et al.
"Augmented microscopy: real-time overlay of bright-field and near-infrared fluorescence images", J. Biomed. Opt. 20(10), 106002 (Oct 06, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.106002


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