Research Papers: Imaging

Laser line illumination scheme allowing the reduction of background signal and the correction of absorption heterogeneities effects for fluorescence reflectance imaging

[+] Author Affiliations
Frédéric Fantoni, Lionel Hervé, Vincent Poher, Jean-Marc Dinten

University Grenoble Alpes, F-38000 Grenoble, France CEA, LETI, MINATEC Campus, 17 rue des Martyrs, Grenoble 38054, France

Sylvain Gioux

Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, ES-200 Boston, Massachusetts 02215, United States

Jérôme I. Mars

University Grenoble Alpes, France CNRS, Gipsa-Lab, 11 Rue des Mathématiques, Saint-Martin-d’Hères 38400, France

J. Biomed. Opt. 20(10), 106003 (Oct 07, 2015). doi:10.1117/1.JBO.20.10.106003
History: Received April 30, 2015; Accepted September 9, 2015
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Abstract.  Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality as it allows to noninvasively monitor the fluorescence targeted tumors located below the tissue surface. Some drawbacks of this technique are the background fluorescence decreasing the contrast and absorption heterogeneities leading to misinterpretations concerning fluorescence concentrations. We propose a correction technique based on a laser line scanning illumination scheme. We scan the medium with the laser line and acquire, at each position of the line, both fluorescence and excitation images. We then use the finding that there is a relationship between the excitation intensity profile and the background fluorescence one to predict the amount of signal to subtract from the fluorescence images to get a better contrast. As the light absorption information is contained both in fluorescence and excitation images, this method also permits us to correct the effects of absorption heterogeneities. This technique has been validated on simulations and experimentally. Fluorescent inclusions are observed in several configurations at depths ranging from 1 mm to 1 cm. Results obtained with this technique are compared with those obtained with a classical wide-field detection scheme for contrast enhancement and with the fluorescence by an excitation ratio approach for absorption correction.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Frédéric Fantoni ; Lionel Hervé ; Vincent Poher ; Sylvain Gioux ; Jérôme I. Mars, et al.
"Laser line illumination scheme allowing the reduction of background signal and the correction of absorption heterogeneities effects for fluorescence reflectance imaging", J. Biomed. Opt. 20(10), 106003 (Oct 07, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.106003


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