Research Papers: Imaging

Swept-source optical coherence tomography powered by a 1.3-μm vertical cavity surface emitting laser enables 2.3-mm-deep brain imaging in mice in vivo

[+] Author Affiliations
Woo June Choi, Ruikang K. Wang

University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States

J. Biomed. Opt. 20(10), 106004 (Oct 08, 2015). doi:10.1117/1.JBO.20.10.106004
History: Received July 2, 2015; Accepted September 15, 2015
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Abstract.  We report noninvasive, in vivo optical imaging deep within a mouse brain by swept-source optical coherence tomography (SS-OCT), enabled by a 1.3-μm vertical cavity surface emitting laser (VCSEL). VCSEL SS-OCT offers a constant signal sensitivity of 105 dB throughout an entire depth of 4.25 mm in air, ensuring an extended usable imaging depth range of more than 2 mm in turbid biological tissue. Using this approach, we show deep brain imaging in mice with an open-skull cranial window preparation, revealing intact mouse brain anatomy from the superficial cerebral cortex to the deep hippocampus. VCSEL SS-OCT would be applicable to small animal studies for the investigation of deep tissue compartments in living brains where diseases such as dementia and tumor can take their toll.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Woo June Choi and Ruikang K. Wang
"Swept-source optical coherence tomography powered by a 1.3-μm vertical cavity surface emitting laser enables 2.3-mm-deep brain imaging in mice in vivo", J. Biomed. Opt. 20(10), 106004 (Oct 08, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.106004


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