Special Section on Optical Medical Imaging Standards

Microfabrication of polydimethylsiloxane phantoms to simulate tumor hypoxia and vascular anomaly

[+] Author Affiliations
Qiang Wu, Zelin Yu, Erbao Dong, Shiwu Zhang

University of Science and Technology of China, Department of Precision Machinery and Precision Instrumentation, 96 Jinzhai Road, Hefei, Anhui 230027, China

Wenqi Ren, Ronald X. Xu

University of Science and Technology of China, Department of Precision Machinery and Precision Instrumentation, 96 Jinzhai Road, Hefei, Anhui 230027, China

The Ohio State University, Department of Biomedical Engineering, 1080 Carmack Road, Columbus, Ohio 43210, United States

J. Biomed. Opt. 20(12), 121308 (Oct 12, 2015). doi:10.1117/1.JBO.20.12.121308
History: Received February 20, 2015; Accepted August 31, 2015
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Abstract.  We introduce a microfluidic approach to simulate tumor hypoxia and vascular anomaly. Polydimethylsiloxane (PDMS) phantoms with embedded microchannel networks were fabricated by a soft lithography process. A dialysis membrane was sandwiched between two PDMS slabs to simulate the controlled mass transport and oxygen metabolism. A tortuous microchannel network was fabricated to simulate tumor microvasculature. A dual-modal multispectral and laser speckle imaging system was used for oxygen and blood flow imaging in the tumor-simulating phantom. The imaging results were compared with those of the normal vasculature. Our experiments demonstrated the technical feasibility of simulating tumor hypoxia and vascular anomalies using the proposed PDMS phantom. Such a phantom fabrication technique may be potentially used to calibrate optical imaging devices, to study the mechanisms for tumor hypoxia and angiogenesis, and to optimize the drug delivery strategies.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Qiang Wu ; Wenqi Ren ; Zelin Yu ; Erbao Dong ; Shiwu Zhang, et al.
"Microfabrication of polydimethylsiloxane phantoms to simulate tumor hypoxia and vascular anomaly", J. Biomed. Opt. 20(12), 121308 (Oct 12, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.12.121308


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