Research Papers: Therapeutic

In vivo studies of ultrafast near-infrared laser tissue bonding and wound healing

[+] Author Affiliations
Vidyasagar Sriramoju, Robert R. Alfano

The City College of New York, Institute of Ultrafast Spectroscopy and Lasers, Department of Physics and Electrical Engineering, 160 Convent Avenue, New York, New York 10031, United States

J. Biomed. Opt. 20(10), 108001 (Oct 14, 2015). doi:10.1117/1.JBO.20.10.108001
History: Received June 18, 2015; Accepted August 28, 2015
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Abstract.  Femtosecond (fs) pulse lasers in the near-infrared (NIR) range exhibit very distinct properties upon their interaction with biomolecules compared to the corresponding continuous wave (CW) lasers. Ultrafast NIR laser tissue bonding (LTB) was used to fuse edges of two opposing animal tissue segments in vivo using fs laser photoexcitation of the native vibrations of chomophores. The fusion of the incised tissues was achieved in vivo at the molecular level as the result of the energy–matter interactions of NIR laser radiation with water and the structural proteins like collagen in the target tissues. Nonthermal vibrational excitation from the fs laser absorption by water and collagen induced the formation of cross-links between tissue proteins on either sides of the weld line resulting in tissue bonding. No extrinsic agents were used to facilitate tissue bonding in the fs LTB. These studies were pursued for the understanding and evaluation of the role of ultrafast NIR fs laser radiation in the LTB and consequent wound healing. The fs LTB can be used for difficult to suture structures such as blood vessels, nerves, gallbladder, liver, intestines, and other viscera. Ultrafast NIR LTB yields promising outcomes and benefits in terms of wound closure and wound healing under optimal conditions.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Vidyasagar Sriramoju and Robert R. Alfano
"In vivo studies of ultrafast near-infrared laser tissue bonding and wound healing", J. Biomed. Opt. 20(10), 108001 (Oct 14, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.108001


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