Research Papers: Imaging

Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion

[+] Author Affiliations
Mike Friedrich

University of Würzburg, Rudolf Virchow Center, Microscopy Group, Bio-Imaging Center, Josef-Schneider-Str. 2, 97080 Würzburg, Germany

Gregory S. Harms

University of Würzburg, Rudolf Virchow Center, Microscopy Group, Bio-Imaging Center, Josef-Schneider-Str. 2, 97080 Würzburg, Germany

Wilkes University, Department of Biology and Physics, Wilkes-Barre, Pennsylvania 18766, United States

J. Biomed. Opt. 20(10), 106006 (Oct 15, 2015). doi:10.1117/1.JBO.20.10.106006
History: Received May 29, 2015; Accepted August 28, 2015
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Abstract.  Planar illumination imaging allows for illumination of the focal plane orthogonal to the imaging axis in various light forms and is advantageous for high optical sectioning, high imaging speed, low light exposure, and inherently deeper imaging penetration into small organisms and tissue sections. The drawback of the technique is the low inherent resolution, which can be overcome by the incorporation of a dual-sheet stimulated emission depletion (STED) beam to the planar illumination excitation. Our initiative is the implementation of STED into the planar illumination microscope for enhanced resolution. We demonstrate some of our implementations. The depletion of STED in the microscope follows an inverse square root saturation for up to 2.5-fold axial resolution improvements with both high and low numerical aperture imaging objectives.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Mike Friedrich and Gregory S. Harms
"Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion", J. Biomed. Opt. 20(10), 106006 (Oct 15, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.106006


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