Special Section on Quantitative Phase Imaging in Biomedicine

Quantitative investigation of red blood cell three-dimensional geometric and chemical changes in the storage lesion using digital holographic microscopy

[+] Author Affiliations
Keyvan Jaferzadeh, Inkyu Moon

Chosun University, Department of Computer Engineering, 309 Pilmun-daero, Dong-gu, Gwangju 501-759, Republic of Korea

J. Biomed. Opt. 20(11), 111218 (Oct 21, 2015). doi:10.1117/1.JBO.20.11.111218
History: Received April 7, 2015; Accepted September 22, 2015
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Abstract.  Quantitative phase information obtained by digital holographic microscopy (DHM) can provide new insight into the functions and morphology of single red blood cells (RBCs). Since the functionality of a RBC is related to its three-dimensional (3-D) shape, quantitative 3-D geometric changes induced by storage time can help hematologists realize its optimal functionality period. We quantitatively investigate RBC 3-D geometric changes in the storage lesion using DHM. Our experimental results show that the substantial geometric transformation of the biconcave-shaped RBCs to the spherocyte occurs due to RBC storage lesion. This transformation leads to progressive loss of cell surface area, surface-to-volume ratio, and functionality of RBCs. Furthermore, our quantitative analysis shows that there are significant correlations between chemical and morphological properties of RBCs.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Keyvan Jaferzadeh and Inkyu Moon
"Quantitative investigation of red blood cell three-dimensional geometric and chemical changes in the storage lesion using digital holographic microscopy", J. Biomed. Opt. 20(11), 111218 (Oct 21, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.111218


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