Research Papers: General

All-plastic miniature fluorescence microscope for point-of-care readout of bead-based bioassays

[+] Author Affiliations
Alessandra Forcucci, Michal Emanuel Pawlowski, Zachary Crannell, Ina Pavlova, Rebecca Richards-Kortum, Tomasz S. Tkaczyk

Rice University, Department of Bioengineering, 6100 Main Street, Houston, Texas 77005, United States

J. Biomed. Opt. 20(10), 105010 (Oct 21, 2015). doi:10.1117/1.JBO.20.10.105010
History: Received June 22, 2015; Accepted September 17, 2015
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Abstract.  A number of new platforms have been developed for multiplexed bioassays that rely on imaging targeted fluorescent beads labeled with different fluorescent dyes. We developed a compact, low-cost three-dimensional printed fluorescence microscope that can be used as a detector for mutiplexed, bead-based assays to support point-of-care applications. Images obtained with the microscope were analyzed to differentiate multiple analytes in a single sample with a comparable limit of detection to commercially available macroscopic assay platforms.

© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Alessandra Forcucci ; Michal Emanuel Pawlowski ; Zachary Crannell ; Ina Pavlova ; Rebecca Richards-Kortum, et al.
"All-plastic miniature fluorescence microscope for point-of-care readout of bead-based bioassays", J. Biomed. Opt. 20(10), 105010 (Oct 21, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.105010


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