Research Papers: Therapeutic

Effect of low-level laser therapy on irradiated parotid glands—study in mice

[+] Author Affiliations
Monique Dossena Acauan, Maria Antonia Zancanaro de Figueiredo, Karen Cherubini, Fernanda Gonçalves Salum

Pontifical Catholic University of Rio Grande do Sul—PUCRS, Oral Medicine Division, São Lucas Hospital, Avenue Ipiranga, 6690, Room 231, CEP: 90610-000, Porto Alegre, RS, Brazil

Ana Paula Neutziling Gomes

Federal University of Pelotas—UFPEL, Oral Pathology Division, Rua Gonçalves Chaves 457, CEP: 96015-560, Pelotas, RS, Brazil

Aroldo Braga-Filho

Pontifical Catholic University of Rio Grande do Sul-PUCRS, Radiotherapy Division, São Lucas Hospital, Brazil

J. Biomed. Opt. 20(10), 108002 (Oct 22, 2015). doi:10.1117/1.JBO.20.10.108002
History: Received March 5, 2015; Accepted September 23, 2015
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Abstract.  The objective of this study was to evaluate the effect of low-level laser therapy (LLLT) on radiotherapy-induced morphological changes and caspase-3 immunodetection in parotids of mice. Forty-one Swiss mice were divided into control, radiotherapy, 2- and 4-J laser groups. The experimental groups were exposed to ionizing radiation in a single session of 10 Gy. In the laser groups, a GaAlAs laser (830 nm, 100 mW, 0.028cm2, 3.57W/cm2) was used on the region corresponding to the parotid glands, with 2-J energy (20 s, 71J/cm2) or 4 J (40 s, 135J/cm2) per point. LLLT was performed immediately before and 24 h after radiotherapy. One point was applied in each parotid gland. The animals were euthanized 48 h or 7 days after radiotherapy and parotid glands were dissected for morphological analysis and immunodetection of caspase-3. There was no significant difference between groups in the immunodetection of caspase-3, but the laser groups had a lower percentage compared to the radiotherapy group. LLLT promoted the preservation of acinar structure, reduced the occurrence of vacuolation, and stimulated parotid gland vascularization. Of the two LLLT protocols, the one using 4 J of energy showed better results.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Monique Dossena Acauan ; Ana Paula Neutziling Gomes ; Aroldo Braga-Filho ; Maria Antonia Zancanaro de Figueiredo ; Karen Cherubini, et al.
"Effect of low-level laser therapy on irradiated parotid glands—study in mice", J. Biomed. Opt. 20(10), 108002 (Oct 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.108002


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