Research Papers: Imaging

Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats

[+] Author Affiliations
Hao Li, Wenzhong Liu

Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States

Hao F. Zhang

Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States

Northwestern University, Department of Ophthalmology, 645 North Michigan Avenue, Chicago, Illinois 60611, United States

J. Biomed. Opt. 20(10), 106010 (Oct 23, 2015). doi:10.1117/1.JBO.20.10.106010
History: Received May 21, 2015; Accepted September 28, 2015
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Abstract.  Rodent models are indispensable in studying various retinal diseases. Noninvasive, high-resolution retinal imaging of rodent models is highly desired for longitudinally investigating the pathogenesis and therapeutic strategies. However, due to severe aberrations, the retinal image quality in rodents can be much worse than that in humans. We numerically and experimentally investigated the influence of chromatic aberration and optical illumination bandwidth on retinal imaging. We confirmed that the rat retinal image quality decreased with increasing illumination bandwidth. We achieved the retinal image resolution of 10μm using a 19 nm illumination bandwidth centered at 580 nm in a home-built fundus camera. Furthermore, we observed higher chromatic aberration in albino rat eyes than in pigmented rat eyes. This study provides a design guide for high-resolution fundus camera for rodents. Our method is also beneficial to dispersion compensation in multiwavelength retinal imaging applications.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Hao Li ; Wenzhong Liu and Hao F. Zhang
"Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats", J. Biomed. Opt. 20(10), 106010 (Oct 23, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.106010


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