Surgical light is important for helping the surgeon easily identify specific tissues during an operation. We propose a spectral reflectance comparison model to optimize the light-emitting diode light spectrum in the operating room. An entropy evaluation method, meant specifically for surgical situations, was developed to evaluate images of biological samples. White light was mixed to achieve an optimal spectrum, and images of different tissues under the light were captured and analyzed. Results showed that images obtained with light with an optimal spectrum had a higher contrast than those obtained with a commercial white light of different color temperatures. Optimized surgical light obtained using this simple and effective method could replace the traditional surgical illumination systems.