Research Papers: General

Mechanism of two-photon excited hemoglobin fluorescence emission

[+] Author Affiliations
Qiqi Sun

Hong Kong University of Science and Technology, Department of Electronic and Computer Engineering, Clear Water Bay, Kowloon, Hong Kong SAR, China

Wei Zheng

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

Jiannong Wang

Hong Kong University of Science and Technology, Department of Physics, Clear Water Bay, Kowloon, Hong Kong SAR, China

Yi Luo

University of Science and Technology of China, Bio-X Division, Hefei National Laboratory for Physical Science at the Microscale, Hefei, Anhui, China

Jianan Y. Qu

Hong Kong University of Science and Technology, Department of Electronic and Computer Engineering, Clear Water Bay, Kowloon, Hong Kong SAR, China

Hong Kong University of Science and Technology, Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Clear Water Bay, Kowloon, Hong Kong SAR, China

J. Biomed. Opt. 20(10), 105014 (Oct 27, 2015). doi:10.1117/1.JBO.20.10.105014
History: Received July 29, 2015; Accepted September 30, 2015
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Abstract.  Hemoglobin, one of the most important proteins in the human body, is composed of “heme” groups (iron-containing rings) and “globins” (proteins). We investigate the two-photon excited fluorescence of hemoglobin and its subunit components (heme and globin). We measure the hemoglobin fluorescence lifetime by using a streak camera of ps resolution and confirm that its lifetime is in femtosecond scale. In the study of the fluorescence properties of heme and globin, the experimental results reveal that heme is the sole fluorophore of hemoglobin. Hemoglobin fluorescence can be effectively excited only via two-photon process, because heme has a centrosymmetric molecular structure and two-photon allowed transition is forbidden for single-photon process and vice versa due to the Laporte parity selection rule.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Qiqi Sun ; Wei Zheng ; Jiannong Wang ; Yi Luo and Jianan Y. Qu
"Mechanism of two-photon excited hemoglobin fluorescence emission", J. Biomed. Opt. 20(10), 105014 (Oct 27, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.10.105014


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