Research Papers: Imaging

Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

[+] Author Affiliations
Junhui Shi, Lihong V. Wang

Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brooking Drive, St. Louis, Missouri 63130-4899, United States

Lidai Wang

Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brooking Drive, St. Louis, Missouri 63130-4899, United States

City University of Hong Kong, Department of Mechanical and Biomedical Engineering, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong SAR, China

Cedric Noordam

Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brooking Drive, St. Louis, Missouri 63130-4899, United States

University of Twente, Optical Sciences, MESA+ Institute for Nanotechnology, P.O. Box 217, 7500 AE Enschede, The Netherlands

J. Biomed. Opt. 20(11), 116002 (Nov 02, 2015). doi:10.1117/1.JBO.20.11.116002
History: Received August 13, 2015; Accepted October 7, 2015
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Abstract.  The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction free, provides a long focal depth, but its side lobes deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic (PA) signals in OR-PAM. We present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in PA imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Junhui Shi ; Lidai Wang ; Cedric Noordam and Lihong V. Wang
"Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field", J. Biomed. Opt. 20(11), 116002 (Nov 02, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.116002


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