Research Papers: Imaging

In situ microscopy using adjustment-free optics

[+] Author Affiliations
Hajo Suhr

Hochschule Mannheim–University of Applied Sciences, Department of Information Technology, Paul-Wittsack-Straße 10, D-68163 Mannheim, Germany

Alois M. Herkommer

University Stuttgart, Institute for Technical Optics, Pfaffenwaldring 9, D-70569 Stuttgart, Germany

J. Biomed. Opt. 20(11), 116007 (Nov 23, 2015). doi:10.1117/1.JBO.20.11.116007
History: Received June 23, 2015; Accepted October 16, 2015
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Abstract.  In the past years, in situ microscopy has been demonstrated as a technique for monitoring the concentration and morphology of moving microparticles in agitated suspensions. However, up until now, this technique can only achieve a high resolution if a certain manual or automated effort is established for continuous precise focusing. Therefore, the application of in situ microscopes (ISMs) as sensors is inhibited in the cases where unattended operation is required. Here, we demonstrate a high-resolution ISM which, unlike others, is built as an entirely rigid construction, requiring no adjustments at all. This ISM is based on a specially designed water immersion objective with numerical aperture=0.75 and a working distance of 15μm. The objective can be built exclusively from off-the-shelf parts and the front surface directly interfaces with the moving suspension. We show various applications of the system and demonstrate the imaging performance with submicron resolution within moving suspensions of microorganisms.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Hajo Suhr and Alois M. Herkommer
"In situ microscopy using adjustment-free optics", J. Biomed. Opt. 20(11), 116007 (Nov 23, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.116007


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In situ microscopy using adjustment-free optics. J Biomed Opt 2015;20(11):116007.
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