JBO Letters

Line-scanning confocal microscopy for high-resolution imaging of upconverting rare-earth-based contrast agents

[+] Author Affiliations
Laura M. Higgins, Margot Zevon, Vidya Ganapathy, Mark C. Pierce

Rutgers University, Department of Biomedical Engineering, 599 Taylor Road, Piscataway, New Jersey 08854, United States

Yang Sheng, Mei Chee Tan

Singapore University of Technology and Design, Engineering Product Development, 8 Somapah Road 487372, Singapore

Richard E. Riman

Rutgers University, Department of Materials Science and Engineering, 607 Taylor Road, Piscataway, New Jersey 08854, United States

Charles M. Roth, Prabhas V. Moghe

Rutgers University, Department of Biomedical Engineering, 599 Taylor Road, Piscataway, New Jersey 08854, United States

Rutgers University, Department of Chemical and Biochemical Engineering, 98 Brett Road, Piscataway, New Jersey 08854, United States

J. Biomed. Opt. 20(11), 110506 (Nov 25, 2015). doi:10.1117/1.JBO.20.11.110506
History: Received August 17, 2015; Accepted November 02, 2015
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Abstract.  Rare-earth (RE) doped nanocomposites emit visible luminescence when illuminated with continuous wave near-infrared light, making them appealing candidates for use as contrast agents in biomedical imaging. However, the emission lifetime of these materials is much longer than the pixel dwell times used in scanning intravital microscopy. To overcome this limitation, we have developed a line-scanning confocal microscope for high-resolution, optically sectioned imaging of samples labeled with RE-based nanomaterials. Instrument performance is quantified using calibrated test objects. NaYF4:Er,Yb nanocomposites are imaged in vitro, and in ex vivo tissue specimens, with direct comparison to point-scanning confocal microscopy. We demonstrate that the extended pixel dwell time of line-scanning confocal microscopy enables subcellular-level imaging of these nanomaterials while maintaining optical sectioning. The line-scanning approach thus enables microscopic imaging of this emerging class of contrast agents for preclinical studies, with the potential to be adapted for real-time in vivo imaging in the clinic.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Laura M. Higgins ; Margot Zevon ; Vidya Ganapathy ; Yang Sheng ; Mei Chee Tan, et al.
"Line-scanning confocal microscopy for high-resolution imaging of upconverting rare-earth-based contrast agents", J. Biomed. Opt. 20(11), 110506 (Nov 25, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.110506


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