Research Papers: General

Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses

[+] Author Affiliations
Aisada Uchugonova, Hans Georg Breunig, Karsten König

Saarland University, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbrücken, Germany

JenLab GmbH, Schillerstrasse 1, 07745 Jena, Germany

Ana Batista

Saarland University, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbrücken, Germany

J. Biomed. Opt. 20(11), 115008 (Nov 30, 2015). doi:10.1117/1.JBO.20.11.115008
History: Received August 20, 2015; Accepted October 19, 2015
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Abstract.  We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Aisada Uchugonova ; Hans Georg Breunig ; Ana Batista and Karsten König
"Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses", J. Biomed. Opt. 20(11), 115008 (Nov 30, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.115008


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