Research Papers: Imaging

Optical coherence tomography spectral analysis for detecting apoptosis in vitro and in vivo

[+] Author Affiliations
Golnaz Farhat, Gregory J. Czarnota

University of Toronto, Department of Medical Biophysics, Faculty of Medicine, 2075 Bayview Avenue, Toronto M4N 3M5, Canada

Sunnybrook Health Sciences Centre, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto M4N 3M5, Canada

Sunnybrook Health Sciences Centre, Radiation Oncology, 2075 Bayview Avenue, Toronto M4N 3M5, Canada

Anoja Giles

Sunnybrook Health Sciences Centre, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto M4N 3M5, Canada

Sunnybrook Health Sciences Centre, Radiation Oncology, 2075 Bayview Avenue, Toronto M4N 3M5, Canada

Michael C. Kolios

Ryerson University, Department of Physics, 350 Victoria Street, Toronto M5B 2K3, Canada

J. Biomed. Opt. 20(12), 126001 (Dec 07, 2015). doi:10.1117/1.JBO.20.12.126001
History: Received July 10, 2015; Accepted November 3, 2015
Text Size: A A A

Abstract.  Apoptosis is a form of programmed cell death characterized by a series of predictable morphological changes at the subcellular level, which modify the light-scattering properties of cells. We present a spectroscopic optical coherence tomography (OCT) technique to detect changes in subcellular morphology related to apoptosis in vitro and in vivo. OCT data were acquired from acute myeloid leukemia (AML) cells treated with cisplatin over a 48-h period. The backscatter spectrum of the OCT signal acquired from the cell samples was characterized by calculating its in vitro integrated backscatter (IB) and spectral slope (SS). The IB increased with treatment duration, while the SS decreased, with the most significant changes occurring after 24 to 48 h of treatment. These changes coincided with striking morphological transformations in the cells and their nuclei. Similar trends in the spectral parameter values were observed in vivo in solid tumors grown from AML cells in mice, which were treated with chemotherapy and radiation. Our results provide a strong foundation from which future experiments may be designed to further understand the effect of cellular morphology and kinetics of apoptosis on the OCT signal and demonstrate the feasibility of using this technique in vivo.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Golnaz Farhat ; Anoja Giles ; Michael C. Kolios and Gregory J. Czarnota
"Optical coherence tomography spectral analysis for detecting apoptosis in vitro and in vivo", J. Biomed. Opt. 20(12), 126001 (Dec 07, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.12.126001


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.