Research Papers: General

Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy

[+] Author Affiliations
Norihiro Honda

Osaka University, Institute for Academic Initiatives, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Yoichiro Kariyama, Hisanao Hazama

Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Takuya Ishii, Yuya Kitajima, Katsushi Inoue, Masahiro Ishizuka, Tohru Tanaka

SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, 1-6-1, Roppongi, Minato-ku, Tokyo 106-6020, Japan

Kunio Awazu

Osaka University, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Osaka University, Graduate School of Frontier Biosciences, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan

Osaka University, The Global Center for Medical Engineering and Informatics, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

J. Biomed. Opt. 20(12), 125001 (Dec 11, 2015). doi:10.1117/1.JBO.20.12.125001
History: Received February 24, 2015; Accepted November 5, 2015
Text Size: A A A

Abstract.  Herein, the optical adequacy of a tumor model prepared with tumor cells grown on the chorioallantoic membrane (CAM) of a chicken egg is evaluated as an alternative to the mouse tumor model to assess the optimal irradiation conditions in photodynamic therapy (PDT). The optical properties of CAM and mouse tumor tissues were measured with a double integrating sphere and the inverse Monte Carlo technique in the 350- to 1000-nm wavelength range. The hemoglobin and water absorption bands observed in the CAM tumor tissue (10 eggs and 10 tumors) are equal to that of the mouse tumor tissue (8 animals and 8 tumors). The optical intersubject variability of the CAM tumor tissues meets or exceeds that of the mouse tumor tissues, and the reduced scattering coefficient spectra of CAM tumor tissues can be equated with those of mouse tumor tissues. These results confirm that the CAM tumor model is a viable alternative to the mouse tumor model, especially for deriving optimal irradiation conditions in PDT.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Norihiro Honda ; Yoichiro Kariyama ; Hisanao Hazama ; Takuya Ishii ; Yuya Kitajima, et al.
"Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy", J. Biomed. Opt. 20(12), 125001 (Dec 11, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.12.125001


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.