The learning curve and interobserver variance of attenuation coefficient () determination from optical coherence tomography (OCT) images were quantified. The of normal and diseased vulvar tissues was determined at five time points by three novice students and three OCT experts who reached consensus for reference. Students received feedback between time points. Eventually, variance in was smaller in images of diseased tissue than in images of normal vulvar tissue. The difference between the consensus and student values was larger for smaller values of . We conclude that routine determination for tissue classification does not require extensive training.