Research Papers: Imaging

Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion

[+] Author Affiliations
Sherazade Aknoun

Aix-Marseille Université, Centre National de la Recherche Scientifique, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France

PHASICS S.A., Parc technologique de Saint Aubin, Route de l’Orme des Merisiers, 91190 Saint Aubin, France

Julien Savatier, Pierre Bon, Frédéric Galland, Lamiae Abdeladim, Serge Monneret

Aix-Marseille Université, Centre National de la Recherche Scientifique, Centrale Marseille, Institut Fresnel UMR 7249, 13013 Marseille, France

Benoit Wattellier

PHASICS S.A., Parc technologique de Saint Aubin, Route de l’Orme des Merisiers, 91190 Saint Aubin, France

J. Biomed. Opt. 20(12), 126009 (Dec 22, 2015). doi:10.1117/1.JBO.20.12.126009
History: Received March 31, 2015; Accepted November 23, 2015
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Abstract.  Single-cell dry mass measurement is used in biology to follow cell cycle, to address effects of drugs, or to investigate cell metabolism. Quantitative phase imaging technique with quadriwave lateral shearing interferometry (QWLSI) allows measuring cell dry mass. The technique is very simple to set up, as it is integrated in a camera-like instrument. It simply plugs onto a standard microscope and uses a white light illumination source. Its working principle is first explained, from image acquisition to automated segmentation algorithm and dry mass quantification. Metrology of the whole process, including its sensitivity, repeatability, reliability, sources of error, over different kinds of samples and under different experimental conditions, is developed. We show that there is no influence of magnification or spatial light coherence on dry mass measurement; effect of defocus is more critical but can be calibrated. As a consequence, QWLSI is a well-suited technique for fast, simple, and reliable cell dry mass study, especially for live cells.

© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Sherazade Aknoun ; Julien Savatier ; Pierre Bon ; Frédéric Galland ; Lamiae Abdeladim, et al.
"Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion", J. Biomed. Opt. 20(12), 126009 (Dec 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.12.126009


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