Research Papers: General

Photobiomodulation reduces oral mucositis by modulating NF-kB

[+] Author Affiliations
Marina Curra, Ana Carolina Amorim Pellicioli, Manoel Sant’Ana Filho, Marco Antonio Trevizani Martins, Manoela Domingues Martins

Universidade Federal do Rio Grande do Sul, School of Dentistry, Department of Oral Pathology, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul 90035-003, Brazil

Nélson Alexandre Kretzmann Filho, Gustavo Ochs, Úrsula Matte

Hospital de Clínicas de Porto Alegre, Department of Gene Therapy, Rua Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90950-000, Brazil

J. Biomed. Opt. 20(12), 125008 (Dec 29, 2015). doi:10.1117/1.JBO.20.12.125008
History: Received August 6, 2015; Accepted November 20, 2015
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Abstract.  The aim of this study was to evaluate NF-kB during 5-fluorouracil (FU)-induced oral mucositis and ascertain whether photobiomodulation (PBM), as a preventive and/or therapeutic modality, influences this transcription factor. Ninety-six male golden Syrian hamsters were allocated into four groups: control (no treatment); PBM therapeutic, PBM preventive, and PBM combined. Animals received an injection of 5-FU on days 0 and 2. On days 3 and 4, the buccal mucosa was scratched. Irradiation was carried out using a 660-nm, 40-mW diode laser at 6  J/cm2 during 6  s/point, 0.24  J/point, for a total dose of 1.44  J/day of application. Animals were euthanized on days 0, 5, 10, and 15 (n=6). Buccal mucosa was removed for protein quantification by Western blot. Clinical analysis revealed that PBM groups exhibited less mucositis than controls on day 10. Control animals exhibited lower levels of NF-kB during mucositis development and healing. The preventive and combined protocols were associated with higher NF-kB levels at day 5; however, the therapeutic group had higher levels at days 10 and 15. These findings suggest that the preventive and/or therapeutic PBM protocols reduced the severity of oral mucositis by activating the NF-kB pathway.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Topics

Mouth ; Wound healing

Citation

Marina Curra ; Ana Carolina Amorim Pellicioli ; Nélson Alexandre Kretzmann Filho ; Gustavo Ochs ; Úrsula Matte, et al.
"Photobiomodulation reduces oral mucositis by modulating NF-kB", J. Biomed. Opt. 20(12), 125008 (Dec 29, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.12.125008


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