Research Papers: General

Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments

[+] Author Affiliations
Irina Schelkanova, Aditya Pandya, Guennadi Saiko, Lidia Nacy, Hannan Babar

Ryerson University, Physics Department, 60 Gould Street, Toronto, M5B 2K3, Canada

Duoaud Shah

UHN Microfabrication Center, Techna Institute for the Advancement of Technology for Health, Toronto M5G 2C4, Canada

Lothar Lilge

UHN Microfabrication Center, Techna Institute for the Advancement of Technology for Health, Toronto M5G 2C4, Canada

University of Toronto, Princess Margaret Cancer Center, Department of Medical Biophysics, Toronto M5G 2C4, Canada

Alexandre Douplik

Keenan Research Center, LKS Knowledge Institute St. Michael Hospital, Toronto M5B 1W8, Canada

Friedrich Alexander University Erlangen-Nuremberg, Erlangen Graduate School in Advanced Optical Technologies, Erlangen 91052, Germany

J. Biomed. Opt. 21(1), 015004 (Jan 14, 2016). doi:10.1117/1.JBO.21.1.015004
History: Received September 24, 2015; Accepted December 8, 2015
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Abstract.  A portable, spatially resolved, diffuse reflectance lensless imaging technique based on the charge-coupled device or complementary metal-oxide semiconductor sensor directly coupled to the fiber optic bundle is proposed for visualization of subsurface structures such as superficial microvasculature in the epithelium. We discuss an experimental method for emulating a lensless imaging setup via raster scanning a single fiber-optic cable over a microfluidic phantom containing periodic hemoglobin absorption contrast. To evaluate the ability of the technique to recover information about the subsurface linear structures, scattering layers formed of the Sylgard® 184 Silicone Elastomer and titanium dioxide were placed atop the microfluidic phantom. Thickness of the layers ranged from 0.2 to 0.7 mm, and the values of the reduced scattering coefficient (μs) were between 0.85 and 4.25  mm1. The results demonstrate that fiber-optic, lensless platform can be used for two-dimensional imaging of absorbing inclusions in diffuse reflectance mode. In these experiments, it was shown that diffuse reflectance imaging can provide sufficient spatial sampling of the phantom for differentiation of 30  μm structural features of the embedded absorbing pattern inside the scattering media.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Irina Schelkanova ; Aditya Pandya ; Guennadi Saiko ; Lidia Nacy ; Hannan Babar, et al.
"Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments", J. Biomed. Opt. 21(1), 015004 (Jan 14, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.1.015004


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