Research Papers: Therapeutic

Intraoperative optical assessment of photodynamic therapy response of superficial oral squamous cell carcinoma

[+] Author Affiliations
Daniel J. Rohrbach

Roswell Park Cancer Institute, Department of Cell Stress Biology, Elm and Carlton Streets, Buffalo, New York 14263, United States

Wright State University, Department of Biomedical, Industrial and Human Factors Engineering, 207 Russ Center, Dayton, Ohio 45435, United States

Nestor Rigual, Hassan Arshad

Roswell Park Cancer Institute, Department of Head and Neck Surgery, Elm and Carlton Streets, Buffalo, New York 14263, United States

Erin C. Tracy, Heinz Baumann

Roswell Park Cancer Institute, Department of Cellular and Molecular Biology, Elm and Carlton Streets, Buffalo, New York 14263, United States

Michelle T. Cooper, Gal Shafirstein, Barbara W. Henderson

Roswell Park Cancer Institute, Department of Cell Stress Biology, Elm and Carlton Streets, Buffalo, New York 14263, United States

Gregory Wilding

Roswell Park Cancer Institute, Department of Biostatistics and Bioinformatics, Elm and Carlton Streets, Buffalo, New York 14263, United States

Mihai Merzianu

Roswell Park Cancer Institute, Department of Pathology and Laboratory Medicine, Elm and Carlton Streets, Buffalo, New York 14263, United States

Ulas Sunar

Roswell Park Cancer Institute, Department of Cell Stress Biology, Elm and Carlton Streets, Buffalo, New York 14263, United States

Wright State University, Department of Biomedical, Industrial and Human Factors Engineering, 207 Russ Center, Dayton, Ohio 45435, United States

State University of New York at Buffalo, Department of Biomedical Engineering, 332 Bonner Hall, Buffalo, New York 14228, United States

J. Biomed. Opt. 21(1), 018002 (Jan 18, 2016). doi:10.1117/1.JBO.21.1.018002
History: Received September 11, 2015; Accepted December 11, 2015
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Abstract.  This study investigated whether diffuse optical spectroscopy (DOS) measurements could assess clinical response to photodynamic therapy (PDT) in patients with head and neck squamous cell carcinoma (HNSCC). In addition, the correlation between parameters measured with DOS and the crosslinking of signal transducer and activator of transcription 3 (STAT3), a molecular marker for PDT-induced photoreaction, was investigated. Thirteen patients with early stage HNSCC received the photosensitizer 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) and DOS measurements were performed before and after PDT in the operating room (OR). In addition, biopsies were acquired after PDT to assess the STAT3 crosslinking. Parameters measured with DOS, including blood volume fraction, blood oxygen saturation (StO2), HPPH concentration (cHPPH), HPPH fluorescence, and blood flow index (BFI), were compared to the pathologic response and the STAT3 crosslinking. The best individual predictor of pathological response was a change in cHPPH (sensitivity=60%, specificity=100%), while discrimination analysis using a two-parameter classifier (change in cHPPH and change in StO2) classified pathological response with 100% sensitivity and 100% specificity. BFI showed the best correlation with the crosslinking of STAT3. These results indicate that DOS-derived parameters can assess the clinical response in the OR, allowing for earlier reintervention if needed.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Daniel J. Rohrbach ; Nestor Rigual ; Hassan Arshad ; Erin C. Tracy ; Michelle T. Cooper, et al.
"Intraoperative optical assessment of photodynamic therapy response of superficial oral squamous cell carcinoma", J. Biomed. Opt. 21(1), 018002 (Jan 18, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.1.018002


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