Research Papers: General

Assessment of asthmatic inflammation using hybrid fluorescence molecular tomography–x-ray computed tomography

[+] Author Affiliations
Xiaopeng Ma, Jaya Prakash, Sarah Glasl, Vasilis Ntziachristos

Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstaedter Landstrasse 1, Neuherberg, D-85746, Germany

Technische Universität München, Chair for Biological Imaging, Ismaninger Street 22, Munich 81675, Germany

Francesca Ruscitti

University of Parma, Department of Biomedical, Biotechnological and Translational Science, via del Taglio 10, Parma, 43126, Italy

Fabio Franco Stellari, Gino Villetti

Corporate Pre-clinical R&D, Chiesi Farmaceutici S.p.A, Largo Francesco Belloli 11/A, Parma, 43122, Italy

J. Biomed. Opt. 21(1), 015009 (Jan 21, 2016). doi:10.1117/1.JBO.21.1.015009
History: Received October 31, 2015; Accepted December 15, 2015
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Abstract.  Nuclear imaging plays a critical role in asthma research but is limited in its readings of biology due to the short-lived signals of radio-isotopes. We employed hybrid fluorescence molecular tomography (FMT) and x-ray computed tomography (XCT) for the assessment of asthmatic inflammation based on resolving cathepsin activity and matrix metalloproteinase activity in dust mite, ragweed, and Aspergillus species-challenged mice. The reconstructed multimodal fluorescence distribution showed good correspondence with ex vivo cryosection images and histological images, confirming FMT-XCT as an interesting alternative for asthma research.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Xiaopeng Ma ; Jaya Prakash ; Francesca Ruscitti ; Sarah Glasl ; Fabio Franco Stellari, et al.
"Assessment of asthmatic inflammation using hybrid fluorescence molecular tomography–x-ray computed tomography", J. Biomed. Opt. 21(1), 015009 (Jan 21, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.1.015009


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