Research Papers: Imaging

Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy

[+] Author Affiliations
Chukwuemeka Okoro

University of Illinois at Urbana–Champaign, PROBE Lab, Department of Electrical and Computer Engineering, 1206 W Green Street, Urbana, Illinois 61801, United States

Kimani C. Toussaint, Jr.

University of Illinois at Urbana–Champaign, PROBE Lab, Department of Mechanical Science and Engineering, 1206 W Green Street, Urbana, Illinois 61801, United States

University of Illinois at Urbana–Champaign, PROBE Lab, Affiliate in the Department of Electrical and Computer Engineering and Bioengineering, 1206 W Green Street, Urbana, Illinois 61801, United States

J. Biomed. Opt. 21(1), 016011 (Jan 26, 2016). doi:10.1117/1.JBO.21.1.016011
History: Received October 6, 2015; Accepted December 28, 2015
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Abstract.  We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chukwuemeka Okoro and Kimani C. Toussaint, Jr.
"Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy", J. Biomed. Opt. 21(1), 016011 (Jan 26, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.1.016011


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