The difference between the 532 and 1064 nm nucleation temperatures is an interesting, and somewhat unexpected, feature of our data that deserves some discussion. Logically, we would not expect the nucleation temperature, that is, the melanosome temperature that would produce cavitation in the absence of irradiation, to vary as a function of the irradiation wavelength. We would, however, expect it to vary with differing pulse durations, as seen in the work of Kelly,11 since the speed of heating and, hence, the speed of melanosome thermal expansion would influence the contribution of tensile stress to the cavitation process. The theoretical study of laser-induced bubble formation in the retina by Gerstman et al.6 states that the nucleation temperature for RPE melanosomes, in situ, should be somewhere between the standard temperature and pressure boiling point, 100°C, and the triple point of water, 374°C, varying with pulse duration and pressure of the surrounding fluid. From the more detailed theoretical study of Sun et al.,10 we see that theoretical calculation of the true nucleation temperature, or even of the granule temperature reached at the threshold radiant exposure for laser cavitation, is highly complex, involving simultaneous solution of many coupled differential equations and consideration of the coupled physical effects of shock wave expansion and bubble formation.