The configuration of the SFDI imaging system is shown in Fig. 1. Sinusoidal patterns were projected using an automated Microsoft PowerPoint presentation, from a DLP projector (XD430U, Mitsubishi) consisting of a 0.55-in. single-chip digital micro mirror device and a 230-W Tungsten Halogen lamp. The RGB color wheels were removed to provide uniform illumination on the sample. The projector was turned on for 10 min before the experiments for stabilizing the source. The diffuse reflected light was captured by a camera (Edmund Optics USB EO-0413M 1/3” CMOS Monochrome, 8 bit, , dynamic range linear) synchronized with the projection system and capable of imaging up to 87 frames per second. The illumination area was , while the FOV captured by the camera was . The tissue samples were placed with respect to the camera in a way to cover most of the FOV to avoid light leakage from the sides. A laser line filter (Thorlabs FL730-10, CW 730 nm, ) was attached to the camera. The specular reflection was minimized by using two crossed linear polarizers (Edmund Optics, polarization efficiency ), and a small angle of incidence (15 deg to the normal).11 Previous studies on spectroscopic characterization of human ovarian tissue have been performed mainly in the wavelength range of 390 to 600 nm by Utzinger et al.12 using reflectance spectroscopy, and 700 to 900 nm by Aguirre et al.4 using photoacoustic tomography, but both studies are limited in providing the absolute quantitative values of tissue absorption and scattering. As an initial pilot study, the wavelength of 730 nm was selected, mainly because of previous extensive knowledge and data of malignant and benign ovarian tissue absorption or vasculature obtained from photoacoustic tomography studies in this wavelength range.4,13 Also, this wavelength was available for comparison of accuracy of phantoms from our previous frequency domain (FD) diffuse light imaging system.14 The overall acquisition time was , which makes the system suitable for ex vivo evaluation of the ovary. To minimize the effect of ambient light, the system was located in a dark room, and the laser line filter attached to the camera accepted only narrowband illumination.