Research Papers: Imaging

Rotational imaging optical coherence tomography for full-body mouse embryonic imaging

[+] Author Affiliations
Chen Wu, Narendran Sudheendran, Manmohan Singh

University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United States

Irina V. Larina, Mary E. Dickinson

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, United States

Kirill V. Larin

University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United States

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, United States

Tomsk State University, Interdisciplinary Laboratory of Biophotonics, 36 Lenin Avenue, Tomsk 634050, Russia

J. Biomed. Opt. 21(2), 026002 (Feb 05, 2016). doi:10.1117/1.JBO.21.2.026002
History: Received November 6, 2015; Accepted January 7, 2016
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Abstract.  Optical coherence tomography (OCT) has been widely used to study mammalian embryonic development with the advantages of high spatial and temporal resolutions and without the need for any contrast enhancement probes. However, the limited imaging depth of traditional OCT might prohibit visualization of the full embryonic body. To overcome this limitation, we have developed a new methodology to enhance the imaging range of OCT in embryonic day (E) 9.5 and 10.5 mouse embryos using rotational imaging. Rotational imaging OCT (RI-OCT) enables full-body imaging of mouse embryos by performing multiangle imaging. A series of postprocessing procedures was performed on each cross-section image, resulting in the final composited image. The results demonstrate that RI-OCT is able to improve the visualization of internal mouse embryo structures as compared to conventional OCT.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chen Wu ; Narendran Sudheendran ; Manmohan Singh ; Irina V. Larina ; Mary E. Dickinson, et al.
"Rotational imaging optical coherence tomography for full-body mouse embryonic imaging", J. Biomed. Opt. 21(2), 026002 (Feb 05, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.2.026002


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