Research Papers: Imaging

High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography

[+] Author Affiliations
Emilie Benoit a la Guillaume, Franck Martins, Fabrice Harms

LLTech, Pépinière Paris Santé Cochin, 29 rue du Faubourg Saint-Jacques, 75014 Paris, France

Claude Boccara

LLTech, Pépinière Paris Santé Cochin, 29 rue du Faubourg Saint-Jacques, 75014 Paris, France

ESPCI ParisTech, PSL Research University, UMR 7587 CNRS, U979 INSERM, Institut Langevin, 1 rue Jussieu, 75005 Paris, France

J. Biomed. Opt. 21(2), 026005 (Feb 08, 2016). doi:10.1117/1.JBO.21.2.026005
History: Received October 8, 2015; Accepted January 13, 2016
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Abstract.  Full-field optical coherence tomography (FF-OCT) is a powerful tool for nondestructive assessment of biological tissue, i.e., for the structural examination of tissue in depth at a cellular resolution. Mostly known as a microscopy device for ex vivo analysis, FF-OCT has also been adapted to endoscopy setups since it shows good potential for in situ cancer diagnosis and biopsy guidance. Nevertheless, all the attempts to perform endoscopic FF-OCT imaging did not go beyond lab setups. We describe here, to the best of our knowledge, the first handheld FF-OCT endoscope based on a tandem interferometry assembly using incoherent illumination. A common-path passive imaging interferometer at the tip of an optical probe makes it robust and insensitive to environmental perturbations, and a low finesse Fabry–Perot processing interferometer guarantees a compact system. A good resolution (2.7  μm transverse and 6  μm axial) is maintained through the long distance, small diameter relay optics of the probe, and a good signal-to-noise ratio is achieved in a limited 100 ms acquisition time. High-resolution images and a movie of a rat brain slice have been recorded by moving the contact endoscope over the surface of the sample, allowing for tissue microscopic exploration at 20  μm under the surface. These promising ex vivo results open new perspectives for in vivo imaging of biological tissue, in particular, in the field of cancer and surgical margin assessment.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Emilie Benoit a la Guillaume ; Franck Martins ; Claude Boccara and Fabrice Harms
"High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography", J. Biomed. Opt. 21(2), 026005 (Feb 08, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.2.026005


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