JBO Letters

Photoacoustic microscopy of arteriovenous shunts and blood diffusion in early-stage tumors

[+] Author Affiliations
Chenghung Yeh

Washington University in St. Louis, Department of Electrical and Systems Engineering, One Brookings Drive, St. Louis, Missouri 63130, United States

Jinyang Liang, Yong Zhou

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, United States

Song Hu

University of Virginia, Department of Biomedical Engineering, PO Box 800759, Charlottesville, Virginia 22908, United States

Rebecca E. Sohn, Jeffrey M. Arbeit

Washington University School of Medicine, Department of Surgery, Urology Division, St. Louis, Missouri 63130, United States

Lihong V. Wang

Washington University in St. Louis, Department of Electrical and Systems Engineering, One Brookings Drive, St. Louis, Missouri 63130, United States

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, United States

J. Biomed. Opt. 21(2), 020501 (Feb 16, 2016). doi:10.1117/1.JBO.21.2.020501
History: Received December 1, 2015; Accepted January 21, 2016
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Abstract.  Angiogenesis in a tumor region creates arteriovenous (AV) shunts that cause an abnormal venous blood oxygen saturation (sO2) distribution. Here, we applied optical-resolution photoacoustic microscopy to study the AV shunting in vivo. First, we built a phantom to image sO2 distribution in a vessel containing converged flows from two upstream blood vessels with different sO2 values. The phantom experiment showed that the blood from the two upstream vessels maintained a clear sO2 boundary for hundreds of seconds, which is consistent with our theoretical analysis using a diffusion model. Next, we xenotransplanted O-786 tumor cells in mouse ears and observed abnormal sO2 distribution in the downstream vein from the AV shunts in vivo. Finally, we identified the tumor location by tracing the sO2 distribution. Our study suggests that abnormal sO2 distribution induced by the AV shunts in the vessel network may be used as a new functional benchmark for early tumor detection.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chenghung Yeh ; Jinyang Liang ; Yong Zhou ; Song Hu ; Rebecca E. Sohn, et al.
"Photoacoustic microscopy of arteriovenous shunts and blood diffusion in early-stage tumors", J. Biomed. Opt. 21(2), 020501 (Feb 16, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.2.020501


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