Research Papers: Imaging

Wide field of view multifocal scanning microscopy with sparse sampling

[+] Author Affiliations
Jie Wang, Jigang Wu

Shanghai Jiao Tong University, University of Michigan–Shanghai Jiao Tong University Joint Institute, Biophotonics Laboratory, 800 Dong Chuan Road, Shanghai 200240, China

J. Biomed. Opt. 21(2), 026008 (Feb 17, 2016). doi:10.1117/1.JBO.21.2.026008
History: Received October 14, 2015; Accepted January 27, 2016
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Abstract.  We propose to use sparsely sampled line scans with a sparsity-based reconstruction method to obtain images in a wide field of view (WFOV) multifocal scanning microscope. In the WFOV microscope, we used a holographically generated irregular focus grid to scan the sample in one dimension and then reconstructed the sample image from line scans by measuring the transmission of the foci through the sample during scanning. The line scans were randomly spaced with average spacing larger than the Nyquist sampling requirement, and the image was recovered with sparsity-based reconstruction techniques. With this scheme, the acquisition data can be significantly reduced and the restriction for equally spaced foci positions can be removed, indicating simpler experimental requirement. We built a prototype system and demonstrated the effectiveness of the reconstruction by recovering microscopic images of a U.S. Air Force target and an onion skin cell microscope slide with 40, 60, and 80% missing data with respect to the Nyquist sampling requirement.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Jie Wang and Jigang Wu
"Wide field of view multifocal scanning microscopy with sparse sampling", J. Biomed. Opt. 21(2), 026008 (Feb 17, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.2.026008


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