Research Papers: General

Structural effects of simvastatin on rat liver tissue: Fourier transform infrared and Raman microspectroscopic studies

[+] Author Affiliations
Sebnem Garip

Istanbul Kemerburgaz University, Department of Medical Biochemistry, Faculty of Medicine, Mahmutbey Dilmenler Caddesi, No: 26, Istanbul 34217, Turkey

Sevgi Haman Bayari

Hacettepe University, Department of Physics Engineering, Faculty of Engineering, Beytepe Campus, Ankara 06800, Turkey

Mete Severcan

Middle East Technical University, Department of Electrical and Electronics Engineering, Faculty of Engineering, Dumlupinar Bulvari, No: 1, Ankara 06800, Turkey

Sherif Abbas

Middle East Technical University, Department of Biological Sciences, Faculty of Arts and Sciences, Dumlupinar Bulvari, No: 1, Ankara 06800, Turkey

Igor K. Lednev, Feride Severcan

University at Albany, Department of Chemistry, State University of New York, Albany, New York 12222, United States

J. Biomed. Opt. 21(2), 025008 (Feb 18, 2016). doi:10.1117/1.JBO.21.2.025008
History: Received November 11, 2015; Accepted January 21, 2016
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Abstract.  Simvastatin is one of the most frequently prescribed statins because of its efficacy in the treatment of hypercholesterolemia, reducing cardiovascular risk and related mortality. Determination of its side effects on different tissues is mandatory to improve safe use of this drug. In the present study, the effects of simvastatin on molecular composition and structure of healthy rat livers were investigated by Fourier transform infrared and Raman imaging. Simvastatin-treated groups received 50  mg/kg/day simvastatin for 30 days. The ratio of the area and/or intensity of the bands assigned to lipids, proteins, and nucleic acids were calculated to get information about the drug-induced changes in tissues. Loss of unsaturation, accumulation of end products of lipid peroxidation, and alterations in lipid-to-protein ratio were observed in the treated group. Protein secondary structure studies revealed significant decrease in α-helix and increase in random coil, while native β-sheet decreases and aggregated β-sheet increases in treated group implying simvastatin-induced protein denaturation. Moreover, groups were successfully discriminated using principal component analysis. Consequently, high-dose simvastatin treatment induces hepatic lipid peroxidation and changes in molecular content and protein secondary structure, implying the risk of liver disorders in drug therapy.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Sebnem Garip ; Sevgi Haman Bayari ; Mete Severcan ; Sherif Abbas ; Igor K. Lednev, et al.
"Structural effects of simvastatin on rat liver tissue: Fourier transform infrared and Raman microspectroscopic studies", J. Biomed. Opt. 21(2), 025008 (Feb 18, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.2.025008


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