Two phantom sets were constructed to characterize PpIX fluorescence in turbid phantoms in the presence of surfactant: one set containing Intralipid 20% (Fresenius Kabi, Uppsala, Sweden) as the scattering agent with no background absorber, and a second set containing both Intralipid and bovine whole blood (Lampire, Ottsville, Pennsylvania) as a background absorber. Each phantom in this set contained 1.5% lipid volume fraction (LVF), while the absorbing set of phantoms also contained 2% blood volume fraction (BVF). PpIX was solubilized using DMSO at a stock concentration of ; dilutions from this stock were used to achieve the desired PpIX concentration in each phantom. In total, 50 phantoms (20 mL each) were constructed to include a range of five PpIX concentrations [(0.1, 0.3, 1, 3, 10) ], each prepared with five different TVF [(0, 0.1, 0.5, 1, 5)%]. Additionally, a phantom set with 1.5% LVF, 2% BVF, and 1 PpIX was sampled with a more granular set of TVF values [(0, 0.1, 0.5, 1, 2, 3, 4, 5)%]. To confirm the stability and reliability of Intralipid as a scatterer and blood as an absorber for fluorescence measurements, two additional sets of phantoms were made varying LVF and BVF at a TVF of 0.1% for a range of PpIX concentrations [(0.1, 0.3, 1, 3 10) ]. This included variation of BVF [(0.5, 1, 2, 3)%] with a fixed LVF of 1.5%, and also variation of LVF [(1, 1.5, 2)%] with a fixed BVF of 2%. Table 1 provides a detailed overview of the combinations of constituents used within each of the phantom sets.