Research Papers: Imaging

Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain

[+] Author Affiliations
Mélanie Pedrazzani, Sakina Benrezzak, Daniele Nutarelli

Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, France

Vincent Loriette, Alexandra Fragola

CNRS, Laboratoire de Physique et d’Étude des Matériaux, ESPCI, 10 Rue Vauquelin, Paris 75005, France

Paul Tchenio

Université Paris-Saclay, Laboratoire Aimé Cotton, CNRS, Université Paris-Sud, ENS Cachan, Orsay Cedex 91405, France

CNRS, Gènes et Dynamiques des Systèmes de Mémoire, Unité de Neurobiologie, ESPCI, 10 Rue Vauquelin, Paris 75005, France

J. Biomed. Opt. 21(3), 036006 (Mar 11, 2016). doi:10.1117/1.JBO.21.3.036006
History: Received November 7, 2015; Accepted February 11, 2016
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Abstract.  We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50  μm deep inside living Drosophila brain.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Mélanie Pedrazzani ; Vincent Loriette ; Paul Tchenio ; Sakina Benrezzak ; Daniele Nutarelli, et al.
"Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain", J. Biomed. Opt. 21(3), 036006 (Mar 11, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.3.036006


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