This method will be faced with challenges if it is combined with auto-sectioning and imaging for the reconstruction of a large brain area. The agents are very viscous, with viscosities of 217, 659, and for sorbitol, sucrose, and fructose, respectively. The high viscosity of the agents and the deformation during the procedure might prevent precise cutting. One possible solution is to ensure intact reconstruction along the -axis by overlapping optical and mechanical sectioning. Another is to optimize the optical clearing method by decreasing the viscosity of the agent. For instance, Pavone’s group used TDE, a low-viscosity agent, to clear the samples and combined with STP to image large-volume tissues,22,23 Myers and Chandrashekar’s group introduced DMSO and an aqueous buffer into sorbitol solution and achieved low viscosity,41 which both provide us with good references. Moreover, the construction of a certain slicing and imaging system is to be developed. As the urgent demand for large-volume tissue reconstruction continues, we believe that a convenient and rapid clearing method combined with sectioning and imaging techniques will provide a new perspective.