Special Section on Tissue and Blood Optical Clearing for Biomedical Applications

Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography

[+] Author Affiliations
Jaeyul Lee, Kanghae Kim, Ruchire Eranga Wijesinghe, Doekmin Jeon, Mansik Jeon

Kyungpook National University, School of Electronics Engineering, College of IT Engineering, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea

Sang Heun Lee

Daegu Veterans Hospital, Department of Otorhinolaryngology, 60 Wolgok-ro, Dalseo-gu, Daegu 42835, Republic of Korea

Jeong Hun Jang

Ajou University, School of Medicine, Department of Otorhinolaryngology-Head and Neck Surgery, 164 World cup-ro, Yeongtong-gu, Suwon, Gyeonggi-do 41944, Republic of Korea

J. Biomed. Opt. 21(8), 081204 (Mar 16, 2016). doi:10.1117/1.JBO.21.8.081204
History: Received December 31, 2015; Accepted February 23, 2016
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Abstract.  The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. Ex vivo mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method, difficulties in imaging of internal cochlea microstructures of mice could be evaded. The obtained results verified that the depth visibility of the decalcified ex vivo samples was enhanced.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Jaeyul Lee ; Kanghae Kim ; Ruchire Eranga Wijesinghe ; Doekmin Jeon ; Sang Heun Lee, et al.
"Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography", J. Biomed. Opt. 21(8), 081204 (Mar 16, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.081204


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