Special Section on Tissue and Blood Optical Clearing for Biomedical Applications

Use of an optical clearing agent to enhance the visibility of subsurface structures and lesions from tooth occlusal surfaces

[+] Author Affiliations
Hobin Kang, Cynthia L. Darling, Daniel Fried

University of California, San Francisco, 707 Parnassus Avenue, San Francisco, California 94143-0758, United States

J. Biomed. Opt. 21(8), 081206 (Apr 12, 2016). doi:10.1117/1.JBO.21.8.081206
History: Received November 16, 2015; Accepted March 15, 2016
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Abstract.  The purpose of this study is to show that optical clearing agents can be used to increase the visibility of deeply penetrating occlusal lesions that have reached the underlying dentin and spread laterally under the enamel. Previous studies have shown that high refractive index fluids can increase the contrast of caries lesions. Extracted teeth with natural occlusal lesions were imaged with optical coherence tomography (OCT) with and without the addition of a transparent vinyl polysiloxane impression material (VPS) currently used in vivo. The relative intensity of the reflectivity from the underlying lesion area for each sample was measured before and after application of the VPS. Lesion presence was confirmed with polarized light microscopy and microradiography. Application of VPS significantly increased (P<0.0001) the integrated reflectivity of subsurface dentinal lesions. This study shows that optical clearing agents can be used to increase the optical penetration and the visibility of subsurface lesions and the dentinal–enamel junction under sound and demineralized enamel in OCT images.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Hobin Kang ; Cynthia L. Darling and Daniel Fried
"Use of an optical clearing agent to enhance the visibility of subsurface structures and lesions from tooth occlusal surfaces", J. Biomed. Opt. 21(8), 081206 (Apr 12, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.081206


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