Research Papers: Imaging

In vivo correlation mapping microscopy

[+] Author Affiliations
James McGrath, Sergey Alexandrov, Hrebesh Subhash

National University of Ireland Galway, Tissue Optics and Microcirculation Imaging Group, National Biophotonics and Imaging Platform, Physics Department, University Road, Newcastle, Galway H91 TK33, Ireland

Peter Owens

National University of Ireland Galway, Centre for Microscopy and Imaging, University Road, Newcastle, Galway H91 TK33, Ireland

Martin Leahy

National University of Ireland Galway, Tissue Optics and Microcirculation Imaging Group, National Biophotonics and Imaging Platform, Physics Department, University Road, Newcastle, Galway H91 TK33, Ireland

RCSI Royal College of Surgeons, 123 St. Stephens Green, Dublin 2 D02 YN77, Ireland

J. Biomed. Opt. 21(4), 046004 (Apr 12, 2016). doi:10.1117/1.JBO.21.4.046004
History: Received December 12, 2015; Accepted March 22, 2016
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Abstract.  To facilitate regular assessment of the microcirculation in vivo, noninvasive imaging techniques such as nailfold capillaroscopy are required in clinics. Recently, a correlation mapping technique has been applied to optical coherence tomography (OCT), which extends the capabilities of OCT to microcirculation morphology imaging. This technique, known as correlation mapping optical coherence tomography, has been shown to extract parameters, such as capillary density and vessel diameter, and key clinical markers associated with early changes in microvascular diseases. However, OCT has limited spatial resolution in both the transverse and depth directions. Here, we extend this correlation mapping technique to other microscopy modalities, including confocal microscopy, and take advantage of the higher spatial resolution offered by these modalities. The technique is achieved as a processing step on microscopy images and does not require any modification to the microscope hardware. Results are presented which show that this correlation mapping microscopy technique can extend the capabilities of conventional microscopy to enable mapping of vascular networks in vivo with high spatial resolution in both the transverse and depth directions.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

James McGrath ; Sergey Alexandrov ; Peter Owens ; Hrebesh Subhash and Martin Leahy
"In vivo correlation mapping microscopy", J. Biomed. Opt. 21(4), 046004 (Apr 12, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.4.046004


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