Special Section on Selected Topics in Biophotonics: Photoacoustic Tomography and Fiber-Based Lasers and Supercontinuum Sources

Inflammation-related alterations of lipids after spinal cord injury revealed by Raman spectroscopy

[+] Author Affiliations
Sandra Tamosaityte, Roberta Galli, Edmund Koch, Gerald Steiner

TU Dresden, Klinisches Sensoring und Monitoring—Klinik für Anästhesiologie und Intensivtherapie, Fetscherstr. 74, 01307 Dresden, Germany

Ortrud Uckermann, Kerim H. Sitoci-Ficici, Robert Later, Gabriele Schackert

TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany

Maria Koch, Matthias Kirsch

TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany

CRTD/DFG-Center for Regenerative Therapies Dresden—Cluster of Excellence, Fetscherstr. 105, 01307 Dresden, Germany

J. Biomed. Opt. 21(6), 061008 (May 04, 2016). doi:10.1117/1.JBO.21.6.061008
History: Received October 15, 2015; Accepted April 11, 2016
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Abstract.  Spinal cord injury (SCI) triggers several lipid alterations in nervous tissue. It is characterized by extensive demyelination and the inflammatory response leads to accumulation of activated microglia/macrophages, which often transform into foam cells by accumulation of lipid droplets after engulfment of the damaged myelin sheaths. Using an experimental rat model, Raman microspectroscopy was applied to retrieve the modifications of the lipid distribution following SCI. Coherent anti-Stokes Raman scattering (CARS) and endogenous two-photon fluorescence (TPEF) microscopies were used for the detection of lipid-laden inflammatory cells. The Raman mapping of CH2 deformation mode intensity at 1440  cm1 retrieved the lipid-depleted injury core. Preserved white matter and inflammatory regions with myelin fragmentation and foam cells were localized by specifically addressing the distribution of esterified lipids, i.e., by mapping the intensity of the carbonyl Raman band at 1743  cm1, and were in agreement with CARS/TPEF microscopy. Principal component analysis revealed that the inflammatory regions are notably rich in saturated fatty acids. Therefore, Raman spectroscopy enabled to specifically detect inflammation after SCI and myelin degradation products.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Sandra Tamosaityte ; Roberta Galli ; Ortrud Uckermann ; Kerim H. Sitoci-Ficici ; Maria Koch, et al.
"Inflammation-related alterations of lipids after spinal cord injury revealed by Raman spectroscopy", J. Biomed. Opt. 21(6), 061008 (May 04, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.061008


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