This report describes how optical images acquired using linearly polarized light can specify the anisotropy of scattering () and the ratio of reduced scattering to absorption (), i.e., . A camera acquired copolarized (HH) and crosspolarized (HV) reflectance images of a tissue (skin), which yielded images based on the intensity () and difference () of reflectance images. Monte Carlo simulations generated an analysis grid (or lookup table), which mapped and into a grid of versus , i.e., and . The anisotropy is interesting because it is sensitive to the submicrometer structure of biological tissues. Hence, polarized light imaging can monitor shifts in the submicrometer (50 to 1000 nm) structure of tissues. The values for forearm skin on two subjects (one Caucasian, one pigmented) were in the range of (24), which is the for 24 measurements on , 470, 524, and 625 nm, which indicated values of (24).