Research Papers: Imaging

In vivo photoacoustic microscopy of human cuticle microvasculature with single-cell resolution

[+] Author Affiliations
Hsun-Chia Hsu, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United States

J. Biomed. Opt. 21(5), 056004 (May 18, 2016). doi:10.1117/1.JBO.21.5.056004
History: Received January 1, 2016; Accepted April 19, 2016
Text Size: A A A

Abstract.  As a window on the microcirculation, human cuticle capillaries provide rich information about the microvasculature, such as its morphology, density, dimensions, or even blood flow speed. Many imaging technologies have been employed to image human cuticle microvasculature. However, almost none of these techniques can noninvasively observe the process of oxygen release from single red blood cells (RBCs), an observation which can be used to study healthy tissue functionalities or to diagnose, stage, or monitor diseases. For the first time, we adapted single-cell resolution photoacoustic (PA) microscopy (PA flowoxigraphy) to image cuticle capillaries and quantified multiple functional parameters. Our results show more oxygen release in the curved cuticle tip region than in other regions of a cuticle capillary loop, associated with a low of RBC flow speed in the tip region. Further analysis suggests that in addition to the RBC flow speed, other factors, such as the drop of the partial oxygen pressure in the tip region, drive RBCs to release more oxygen in the tip region.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Hsun-Chia Hsu ; Lidai Wang and Lihong V. Wang
"In vivo photoacoustic microscopy of human cuticle microvasculature with single-cell resolution", J. Biomed. Opt. 21(5), 056004 (May 18, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.5.056004


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.