Research Papers: Imaging

Nonlinear optical microscopy improvement by focal-point axial modulation

[+] Author Affiliations
Mahdi Mozdoor Dashtabi, Reza Massudi

Shahid Beheshti University, G.C., Laser and Plasma Research Institute, Evin, Tehran 1983969411, Iran

J. Biomed. Opt. 21(5), 056006 (May 26, 2016). doi:10.1117/1.JBO.21.5.056006
History: Received January 18, 2016; Accepted April 27, 2016
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Abstract.  Among the most important challenges of microscopy—even more important than the resolution enhancement, especially in biological and neuroscience applications—is noninvasive and label-free imaging deeper into live scattering samples. However, the fundamental limitation on imaging depth is the signal-to-background ratio in scattering biological tissues. Here, using a vibrating microscope objective in conjunction with a lock-in amplifier, we demonstrate the background cancellation in imaging the samples surrounded by turbid and scattering media, which leads to more clear images deeper into the samples. Furthermore, this technique offers the localization and resolution enhancement as well as resolves ambiguities in signal interpretation, using a single-color laser. This technique is applicable to most nonlinear as well as some linear point-scanning optical microscopies.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Mahdi Mozdoor Dashtabi and Reza Massudi
"Nonlinear optical microscopy improvement by focal-point axial modulation", J. Biomed. Opt. 21(5), 056006 (May 26, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.5.056006


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